Scar

Part:BBa_K5384003:Design

Designed by: Si Cheng   Group: iGEM24_HBUT-Wuhan   (2024-08-24)


Asp-Pro (acid sensitive site)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Under acidic conditions, specific structural changes or chemical reactions may occur at this site. The presence of such an acid-sensitive site may affect the overall structure and stability of the protein. It can be used to design acid-sensitive drug delivery systems. This method is easy to operatte and cheap.

Source

The ASP-PRO acid-sensitive site refers to the region composed of aspartate (ASP) and proline (PRO) in a specific protein or polypeptide sequence.We learned it from literature:XU Xuejiao. Construction of prokaryotic and eukaryotic secretion expression systems of cationic antimicrobial peptides[D]. Anhui:Anhui University,2015.

References

1.ZHAO Huan-le, LIANG Ju, WU Wen-lan, LI Jun-bo. Mechanism and application of acid-sensitive peptides in drug delivery. Acta Pharmaceutica Sinica, 2019, 54(3): 440-447. 2.Xu Xuejiao. Construction of prokaryotic and eukaryotic secretion expression systems of cationic antimicrobial peptides[D].Anhui University,2015.